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SHORT COMMUNICATION
Year : 2013  |  Volume : 4  |  Issue : 2  |  Page : 6

Comparison between two methods of glycosylated hemoglobin estimation among patients with sickle cell trait and diabetes


Department of Medicine, Al-Faiha Diabetes Endocrine and Metabolism Center (FDEMC) Chair Diabetes, Endocrine and Metabolism Division, Basrah College of Medicine, Hattin P.O Box: 142, Basrah - 61013

Correspondence Address:
A A Mansour
Professor of Medicine, Department of Medicine, Al-Faiha Diabetes Endocrine and Metabolism Center (FDEMC). Chair Diabetes, Endocrine and Metabolism Division, Basrah College of Medicine, Hattin P.O Box: 142, Basrah - 61013, Iraq

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Source of Support: None, Conflict of Interest: None


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The aim of this study was to assess the problem of using one non-certified method of HbA1c estimation when used in patients with diabetes with sickle cell trait. Plasma of those who were found to have sickle cell trait on routine HbA1c using automated HPLC instrument were submitted to a quantitative colorimetric method based on turbidimetric immunoassay for the determination of HbA1c in the whole blood. The total enrolled patients were 205. Mean HbA1c by HPLC method was 9.2 ± 2.2% and by the turbidimetric immunoassay method, it was 5.6 ± 1.4%. Those with HbA1c < 7% by turbidimetric immunoassay constituted 87.8%, which is far higher than the figure produced by HPLC method where it was 16.1% only. About 91.8% of the patients had HbA1c higher by 1 - 6.9% using the HPLC method. Because of the common prevalence of sickle-cell trait in Basrah, use of the turbidimetric immunoassay method for the estimation of HbA1c in patients with diabetes should be completely prohibited.


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